Antioxidant Supplements Prevent Oxidation of Cysteine/Cystine Redox in Patients with Age-Related Macular Degeneration

Publication Type
Journal Article
Year of Publication
Moriarty-Craig, S; Adkison, J; Lynn, M; Gensler, G; Bressler, S; Jones, D; Sternberg, P
Am J Ophthalmol
Start Page
Date Published
Aged; Antioxidants; Ascorbic Acid; Chromatography High Pressure Liquid; Copper; Cysteine; Cystine; Dietary Supplements; Female; Glutathione; Macular Degeneration; Male; Oxidation-Reduction; Randomized Controlled Trials as Topic; Vitamin E; Zinc Oxide
{PURPOSE: Determine whether antioxidant supplements alter the plasma glutathione and/or cysteine redox potential in age-related macular degeneration (AMD) patients. DESIGN: This was an ancillary study to the Age-Related Eye Disease Study (AREDS), where subset of AREDS subjects at two sites were studied at two time points, an average of 1.7 and 6.7 years after enrollment. METHODS: Plasma glutathione (GSH), glutathione disulfide (GSSG), cysteine (Cys), and cystine (CySS) were measured by high-performance liquid chromatography, and redox potentials of GSH/GSSG (E(h) GSH) and Cys/CySS (E(h) Cys) were calculated. The means of the metabolites and redox potentials were compared by repeated-measures analysis of variance for subjects receiving antioxidants and those not receiving antioxidants. RESULTS: At the first blood draw, the means for the antioxidant group (n = 153) and no antioxidant group (n = 159) were not significantly different for any of the metabolites or redox potentials. At the second draw, the GSH parameters were not significantly different between the antioxidant (n = 37) and no antioxidant (n = 45) groups; however, mean Cys was significantly higher in the antioxidant group (9.5 vs 7.2 micromol/l