Vaccine-related major cutaneous reaction size correlates with cellular-mediated immune responses after tularaemia immunisation.
2021
Journal Article
Authors:
Salerno-Gonçalves, R.;
Chen, W.H.;
Mulligan, M.J.;
Frey, S.E.;
Stapleton, J.T.;
Keitel, W.A.;
Bailey, J.;
Sendra, E.;
Hill, H.;
Johnson, R.A.;
Sztein, M.B.
Secondary:
Clin Transl Immunology
Volume:
10
Pagination:
e1239
Issue:
1
PMID:
33505681
URL:
https://pubmed.ncbi.nlm.nih.gov/33505681/
Keywords:
Human; T cells; take; tularaemia; Vaccination
Abstract:
Objectives: , the causative agent of tularaemia, is an exceptionally infectious bacterium, potentially fatal for humans if left untreated and with the potential to be developed as a bioweapon. Both natural infection and live-attenuated vaccine strain (LVS) confer good protection against tularaemia. LVS vaccination is traditionally administered by scarification, and the formation of a cutaneous reaction or take at the vaccination site is recognised as a clinical correlate of protection. Although previous studies have suggested that high antibody titres following vaccination might serve as a useful surrogate marker, the immunological correlates of protection remain unknown.Methods: We investigated the host T-cell-mediated immune (T-CMI) responses elicited following immunisation with LVS vaccine formulated by the DynPort Vaccine Company (DVC-LVS) or the United States Army Medical Research Institute of Infectious Diseases (USAMRIID-LVS). We compared T-CMI responses prompted by these vaccines and correlated them with take size.Results: We found that both LVS vaccines elicited similar T-CMI responses. Interestingly, take size associated with the T cells' ability to proliferate, secrete IFN-γ and mobilise degranulation, suggesting that these responses play an essential role in tularaemia protection.Conclusions: These results renew the appreciation for vaccination through the scarification as a prime route of inoculation to target pathogens driving specific T-CMI responses and provide further evidence that T-CMI plays a role in protection from tularaemia.
