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Studies of a Prophylactic HIV-1 Vaccine Candidate Based on Modified Vaccinia Virus Ankara (MVA) with and without DNA Priming: Effects of Dosage and Route on Safety and Immunogenicity


Journal Article

Peters, B.S.; Jaoko, W.; Vardas, E.; Panayotakopoulos, G.; Fast, P.; Schmidt, C.; Gilmour, J.; Bogoshi, M.; Manyonyi, O.; Dally, L.; Klavinskis, L.; Farah, B.; Tarragona, T.; Bart, P.A.; Robinson, A.; Pieterse, C.; Stevens, W.; Thomas, R.; Barin, B.; Bwayo, J.[...]





Adolescent; Adult; AIDS Vaccines; Epitopes- T-Lymphocyte; Female; HIV-1; Immunization- Secondary; Injections- Intradermal; Injections- Intramuscular; Injections- Subcutaneous; Interferon-gamma; Leukocytes- Mononuclear; Male; Middle Aged; Vaccination

BACKGROUND: Two parallel studies evaluated safety and immunogenicity of a prophylactic HIV-1 vaccine in 192 HIV-seronegative, low-risk volunteers. Modified vaccinia virus Ankara (MVA) and plasmid DNA (pTHr) expressed HIV-1 clade A gag p24 and p17 fused to a string of 25 overlapping CD8+ T cell epitopes (HIVA). METHODS: These studies compared intramuscular, subcutaneous, and intradermal MVA at dosage levels ranging from 5x10(6)-2.5x10(8) pfu. In Study IAVI-010, DNA vaccine was given as a prime at months 0 and 1, followed by MVA as a boost at months 5 and 8. In Study IAVI-011, MVA alone was given at months 0 and 2. Regular safety monitoring was performed. Immunogenicity was measured by the interferon (IFN)-gamma ELISPOT assay on peripheral blood mononuclear cells (PBMC). RESULTS: No serious adverse events were attributed to either vaccine; most adverse events were mild or moderate, although MVA resulted in some severe local reactions. Five vaccine recipients had at least one positive IFN-gamma ELISPOT response, but none were sustained. CONCLUSION: This HIV-1 vaccine candidate was in general safe and well-tolerated. Local reactions were common, but tolerable. Detectable immune responses were infrequent.

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